Magnetic Tweezers of all flavors

Conventional Magnetic Tweezers

Conventional magnetic tweezers can apply both force and torque to biological molecules. Advantages of the conventional magnetic tweezers include particularly straightforward control of the supercoiled state of DNA, facile extension to parallel measurement of multiple molecules and to integration with fluorescence measurements, as well as a simplicity and robustness of the experimental configuration. Read more

Magnetic Torque Tweezers

magnetic_torque_tweezerRecently our lab has developed a number of new magnetic tweezers approaches, including the magnetic torque tweezers. These tweezers allow one to directly measure the torque stored in biological molecules. Read more

Electromagnetic Torque Tweezers

EMTTWhere in classic magnetic tweezers (MT) and magnetic torque tweezers (MTT) the force and torque applied to the magnetic bead are intrinsically coupled, the electromagnetic torque tweezers (eMTT) concept combines permanent magnets with electromagnets to independently control the force and torque. Read more

Freely Orbiting Magnetic Tweezers

zero_torque_rec_A_final_versionFreely-orbiting magnetic tweezers (FOMT) allow the measurement of equilibrium fluctuations and changes in the twist of tethered nucleic acid molecules. Using a precisely aligned vertically oriented magnetic field, FOMT enable tracking of the rotation angle from straight forward (x,y)-position tracking and permits the application of calibrated stretching forces, without biasing the tether’s free rotation. We have used FOMT to measure the force-dependent torsional stiffness of DNA from equilibrium rotational fluctuations and to follow the assembly of recombination protein A filaments on DNA. Read more